Sensitivity of human and murine hemopoietic precursor cells to chemotherapeutic agents assessed in cell culture.

The sensitivity of human and murine cate that human and murine hemopoietic hemopoietic precursor cells to 1 ,3 bis cells may differ in their intrinsic sensitivity (2 chloroethyl)-1-nitrosourea (BCNU), nitodrugs. The hematologic toxicity of some trogen mustard, 5-fluorouracil, and melchemotherapeutic agents cannot be cxphalan was assessed in culture using trapolated directly from mouse to man. colony assay techniques. The results mdiM OUSE MODEL SYSTEMS are frequently used to assess the effects ofchemotherapeutic agents prior to clinical trial. This usage is based on the assumption that findings in the mouse are applicable to man. This ssumption can be tested since very similar assay procedures are available for human and murine granulopoietic progenitors in culture (CFU-C). Accordingly, we used the cell culture method to assess the sensitivity of human and murineCFU-C to melphalan, nitrogen mustard, 5-fluorouracil (5 FU), and 1,3 bis (2 chloroethyl)l-nitrosourea (BCN U). Significant species differences were encountered.